Canine distemper is a highly prevalent, contagious virus caused by the paramyxovirus. Normally the mortality in juveniles is higher than in adults. CDV can be prevented by vaccination and CDV antibody detection is used to evaluate the immune status of a dog after vaccination. A specific antigen to CDV is conjugated with gold particles and another specific antigen immobilizes the band on the nitrocellulose membrane. If a distemper is present, the antibody labeled with gold binds it, forming an antibody-antigen complex. As the mixture flows along the membrane, the membrane, the complex is captured by the antibody immobilized in the test line (T) of the membrane, producing a visible black/gray color band. Another gold-conjugated reagent is captured by the antibody immobilized in the control line (C) of the membrane.

One Step Canine Distemper Virus Antibody Test is a rapid immunochromatographic assay that detects antibody against canine distemper virus in canine serum, plasma or eye discharge.