Exhibition

Fully Automated Protein Synthesis/purification

Fully Automated Protein Synthesis/purification


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
South Korea
Brand name
ExiProgen™
Payment Terms
Negotiable
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
protein purification, protein synthesis system, nucleic acid extraction system, protein synthesis/purification
Category
Other Extracts , Chemical Reagents & Products , Amino Acids and Coenzymes

BIONEER CORPORATION

Membership
VIP
Country / Year Established
South Korea South Korea /
Business type
Others
Verified Certificate

16

TRADE TOWER

DUNS

Product name Fully Automated Protein Synthesis/purification Certification -
Category Other Extracts
Chemical Reagents & Products
Amino Acids and Coenzymes
Ingredients -
Keyword protein purification , protein synthesis system , nucleic acid extraction system , protein synthesis/purification Unit Size 320.0 * 500.0 * 535.0 mm
Brand name ExiProgen™ Unit Weigh 27 kg
origin South Korea Stock -
Supply type - HS code 902580

Product Information

ExiProgen™ is an instrument that automatically synthesizes protein from DNA. This product performs In-vitro transcription and translation automatically when loaded with DNA coding a protein and a protein synthesis kit. By purifying this protein through affinity chromatography method, more than 90% pure protein can be obtained within 6 hours and up to 16 species can be produced at one time. In addition, highly expressed affinity tagged proteins can be purified in cell lines with high purity.                 

  • Built-in optimized nucleic acid and protein synthesis protocol

    Simplified usage and reproducible results with built-in nucleic acid extraction and protein purification protocols optimized for various samples (whole blood, tissue, cell, bacteria, plant, etc.)

  • High stability

    Minimized protein denaturation by installing cooling reaction block inside the instrument keeping the temperature of elution tube rack below 10°C

  • High-pure nucleic acid extraction & protein purification

    Quick performance of nucleic acid extraction and protein purification with great purity and efficiency by attaching magnetic particles to the bottom of each well equally and rapidly by magnetic blocks attached to the instrument
    Increased elution efficiency allowing complete evaporation of alcohols by its heating block (Korean Patent No. 10-1025135)

  • Contamination prevention device

    Substantial blocking of cross-contamination, which may occur in other wells by the aerosols generated from the tip, through the sophisticated designs drop-contamination prevention device located at the bottom of the filter tip

  • Automatic UV sterilization Function

    Sterilization to prevent infection for the users by the samples through the UV lamp installed inside the instrument

  • Easy-to-use

    User-friendly UI capable of checking the experimental progress rate real-time without the need of seperate PC by equipping with a 13.3-inch touch screen on the front of the device

  • Excellent precision

    Minimized error range, that may occur during the automatic dispensing of the solution, through installment of an adjustable syringe motor up to 0.1 mm capable of stably distributing in a volume of 25 ㎕ ~ 1000 ㎕ for application to various protocols and obtaining of experimental results

Specifications

Dimensions (cm)32 (W) x 53.5 (D) x 50 (H)
Weight (kg)27
Temperature range15-35℃
Humidity range20-80%, no condensation
Operating systemStandalone
User interface320 x 240 touch screen graphic LCD
Input voltage100 – 240 VAC
Frequency50 / 60 Hz
UV sterilization15 minute cycle
CommunicationsTCP/IP
Heat block40-90℃

Function

DNA/RNA ExtractionO
Protein ExpressionO
Protein PurificationO
TFT Touch ScreenO
Sample Number16 samples
UV sterilizationO
Heating & magnetic blockO
Cartridge Contamination shieldO
TCP/IP network connectionNumerous equipment can be connected
Cooling system for sample storeO
Automatic dispensingO
Power supply24 VDC, 7.5 A(180 W)

* Specifications of this product are subject to change without notice to improve product performance.

Experimental data

1Protein expression and purification

  • DNA concentration used: 6 - 10 μg (purity > 1.8)
  • Protocol number : 902
  • Total run time : < 6 h
  • yield : <100 μg

Experimental Process


SDS-PAGE analysis
<Simultaneous expression/purification of the same protein (GFP) 16 wells>
Figure 1. GFP protein (His-tagged protein)'s expression/purification with ExiProgen
M; AccuLadder™ Protein Size Maker (Low),
E; expression sample
P; purification sample
<Simultaneous expression/purification of different kinds of proteins>
Figure 2. His-tagged proteins expression/purification with ExiProgen
M; AccuLadder™ Protein Size Maker (Low)
E; expression sample,
P; purification sample


2Total RNA Extraction From Tissue or cultured mammalian cell

  • Sample: 15 ㎎ (rat liver tissue) or 1 X 106 cells (HeLa)
  • Protocol number: 202
  • Average yield: 25 ㎍ (rat liver tissue), 10 ㎍ (HeLa)
  • Average RQS: 9.5 over

RQS(RNA quality score): The quality of the extracted RNA is expressed as a numerical value, the height and width of each rRNA peak, and the peak of degraded RNA fragments are comprehensively calculated and displayed. It is the same concept as the RNA integrity number (RIN) developed by Agilent. It is usually expressed as a value from 0 to 10. The closer to 10, the better the quailty of RNA.

Experimental Process

ExiProgen

Analysis results of Total RNA extracted from tissues of Rat



Figure 3. Since each subunit of rRNA is well observed, it can be confirmed that RNA was intactly extracted without decomposition of RNA during total RNA extraction process.



Results of Real-Time PCR using Total RNA extracted from tissues of Rat



Figure 4.Results of real time RT-PCR using AccuPower® RocketScrpt ™ RT PreMix(K-2101, Bioneer), AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block(A-2060)



Analysis results of total RNA extracted from HeLa cell



Figure 5. Total RNA was extracted from HeLa cell (1 X 106 cells) and analyzed for quality of extracted RNA using LabChip GX (Caliper Life Science). As each subunit of rRNA is well observed, intact RNA was extracted without RNA degradation during total RNA extraction. Also, the average yield was 10 ㎍ and the average RQS was 9.5 or more.



Results of real-time PCR using total RNA extracted from HeLa cell



Figure 6. After the total RNA extraction from HeLa cell (1 X 106 cells), it is results of real-time RT-PCR using AccuPower® RocketScrpt ™ RT PreMix (K-2101, Bioneer), AccuPower® DualStar ™ qPCR PreMix (K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060)



3Genomic DNA from cultured mammalian cell (HeLa)

  • Sample volume: 1X106 cells
  • Protocol number: 107
  • Total prep time: about 1hr 30min.
  • Average yield: 4-8 μg

Experimental Process



Agarose gel analysis

ExiProgen

Figure 7. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).



4Genomic DNA from bacteria (E.coli)

  • Sample volume: 1X109 cells
  • Protocol number: 109
  • Total prep time: about 1hr 40min.
  • Average yield: 8-12 μg

Experimental Process



Agarose gel analysis


Figure 8. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X109 cells of E.coli cells and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



5Genomic DNA from tissue (bovine skeletal muscle)

  • Sample volume: ~25 mg
  • Protocol number: 102
  • Total prep time: about 1hr 30min.
  • Average yield: Up to 20 µg

Experimental Process



Agarose gel analysis


Figure 9. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25 mg of bovine tissues and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



6Genomic DNA from plant tissue (spinach leaf)

  • Sample volume: ~ 100 mg
  • Protocol number: 104
  • Total prep time: about 1 hr
  • Average yield: Up to 5 µg

Experimental Process



Agarose gel analysis


Figure 10. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

 

B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Negotiable Shipping time Negotiable

BIONEER CORPORATION

Country / Year Established
South Korea South Korea /
Membership
VIP
Business type
Others

16

TRADE TOWER

DUNS

President
PARK, HAN-OH
Address
49-3, Munpyeong-dong, Daedoek-gu, Daejeon
Product Category
Pharmaceuticals
No. of Total Employees
101-500
Company introduction
Bioneer is a leading supplier of synthetic DNA, providing high-quality custom oligos at affordable prices. Recent improvements in oligonucleotide synthesis chemistry has led to the production of higher quality oligonucleotides at lower cost. In addition, we have implemented in-house production of raw materials along with our proprietary DNA synthesis system, called 384 parallel super DNA synthesizer, and BioRP oligo purification technology (patent pending). Furthermore, we have recently completed a state-of-the-art oligo manufacturing facility capable of producing over 45,000 oligos /day. Such oligo production capacity enables us to meet above and beyond the needs of our customers.
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