Clean-Q Real time hygiene monitoring system

Clean-Q is..

Clean-Q is a portable ATP measuring device. ATP is an acronym for Adenosine Tri-Phosphate, and every living organism (animals, plants, microorganism, yeast, mold, etc.,) has ATP and uses ATP as energy source for life activities. In other words, ATP can be an indicator for the existence of living organism. Therefore, ATP quantity is proportionate to the quantity of pollutants including bacteria.

Correlation of the number of Bacteria and ATP quantity

The graph shows the cprrelation of measured ATP quantity (unit :RLU). according to the number of bacteria in knife (12cm2). The more bacteria (CFU), the more light (unit : RLU). Title : The confirmation of adequacy of ATP Luminometer as a sanitation monitoring tool during processing in food service facilities Organization : Department of food and nutrition, Changwon national university, technology institute of Korea 3M, Support unit of HACCP of Korea health industry develop-ment institute Authors : Kim Yangsuk, Moon Haegyeong, Gang Seongil, Nam Eunjeong Source : The society of Korea food and nutrition science 39.11.1719~1723(2010)

General measurment example in our life

Wet towel hygiene status measured by Clean-Q

When measuring the pollution of wet towel, it was clean right after opening.
After washing hands, it became rapidly polluted.

- Never clean your mouth with the used towel.

Comparison hygiene status value before and after cleaning with Clean-Q

We compared the status of before and after cleaning with Clean-Q, selecting things closely related in our life.
You can confirm the number decreased after cleaning by 1/10

The recommended value of Clean-Q measurement

• Things directly related to food (for example, spoon, dish, etc.,) : safe 75 or lower
• Things directly contactinf skin (for example, hands, phones, etc.,) : safe 150 or lower
• Things that do not directly contact skin but need management (for example, sink drainage,etc.,) : safe 250 or lower

- The values above are recommended by Teltron and subject to change by cases and environment.
- The measurement for beneficial bacteria such as in kimchi and yogurt may not be substantially effective.

Clean-Q which has the least error rate among the products of same kinds.

Experiment method

1. Purchase undiluted solution of ATP.
2. Dilute the solution with PBS by 1pmol density
3. Soak reagent in 10 μℓ of the solution, using micro pipette.
4. Measure with terminals.

• Clean-Q: 2.6%
  
• Company A : 15.7%
  
• Company B: 8.4%
  
• Company C: 16.2%

Since the same ATP density is expressed as different RLU value in each company, % deveaion from average valuea are represented to ensure fairness

The correlation of the number of actual bacteria and measurement value of Clean-Q

Experiment method

1. Take unidentified bacteria from human hands.
   
2. Add 3% of sugar in the water where 3 people wash their hands, and leave it for 24 hours in normal temperature.
   
3. Incubate it with petri film of 3m for 48 hours and identify CFU of 1mℓ
   
4. Repeatedly measure the average value 10 times, using Clean-Q, and find out RLU of 1mℓ.
   
5. Calculate the correlation of RLU and CFU

Incubation method with petri film

1. Take 1mℓ after diluting the incubated bacteria by 1,000 times, 10,000 times and 100,000 times, and inoculate the petrifilm with the germs.
2. Incubate 9 sheets of petri films, 3 sheets for 3 kinds accirding to the degree of dilutions in incubator for 48 hours.
3. Count the germs according to the degree of dilution and take their average.
4. If there are more germs, count their number in a cell and multifly by 20 (source : department of biomedicine, Baejae university)

Caution : The amount of ATP within bacteria is different form each other. Therefor it may be meaningless to estimate the quntity of specification bacteria

Measured resits of 6 main food poisonings

Measuring capacity of 6 main food poisonings

6 main food poisonings - Staphylococcus, Colon Bacillus, Listeria, Vibrio, 0-157, Salmonella.
Measured value (RLU) graph of Clean-Q according to the number of bacteria in each food poisoning (Source : department of biomedicine, Baejae university)

Specification

How to use Clean-Q

1. Surub evenly the area of 10cm x 10cm for mea-surement
2. Break the upper part of the tube and press to let it flow well.
3. Press "Measurement" and Shake for 10 seconds.
4. Put the reagent tube in the terminal, close cover and measure by pressing "Start"