Streptomycin ELISA Test Kit

Streptomycin ELISA Test Kit


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Keyword
reagen
Category
Pet Products
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REAGEN LLC

Country / Year Established
U.S.A U.S.A /
Business type
Others
Verified Certificate

12

Product name Streptomycin ELISA Test Kit Certification -
Category Pet Products Ingredients -
Keyword reagen Unit Size -
Brand name - Unit Weigh -
origin Stock -
Supply type - HS code -

Product Information

 Streptomycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of streptomycin and dihydrostreptomycin in feed, honey, kidney, liver, meat (beef, chicken and pork), milk, serum and urine. The unique features of the kit are:

§ Rapid (10 - 30 minutes), and organic reagent-free extraction methods for various samples with high recovery (80 - 95%).

§ High sensitivity (0.5 ng/g or ppb) and low detection limit (5 ng/g or ppb for meat and milk).

§ A quick ELISA assay (less than 2 hours regardless of number of samples).

§ High reproducibility.

 

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

 

Kit Contents, Storage and Shelf Life

REAGEN™ Streptomycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents

Amount

Storage

Streptomycin-coated Microtiter Plate

1 x 96-well plate (8 wells x 12 strips)

2-8°C

Streptomycin Standards:

 Negative control (white cap tube)

0.5 ng/mL (yellow cap tube)

1.0 ng/mL (orange cap tube)

2.5 ng/mL (pink cap tube)

  5.0 ng/mL (purple cap tube)

10 ng/mL (blue cap tube)

1000 ng/mL (spiking, red cap tube)

 

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

2-8°C

Streptomycin Antibody #1

15 mL

2-8°C *

100X HRP-Conjugated Antibody #2

250 mL

2-8°C *

Antibody #2 Diluent

20 mL

2-8°C

200X Sample Extraction Buffer

25 mL

2-8°C

20X Wash Solution

28 mL

2-8°C

Stop Buffer

20 mL

2-8°C

TMB Substrate

12 mL

2-8°C

Sample Balance Buffer

0.5 mL

2-8°C

10X PBS

25 mL

2-8°C

*If you are not planning to use the kit for over 3 months, store Streptomycin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.

 

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REAGEN LLC

Country / Year Established
U.S.A U.S.A /
Business type
Others

12

President
MR DENG
Address
102 Commerce Dr. Unit 8, Moorestown, NJ 08057
Product Category
Machinery & Parts
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