Liposomes have long been used as drug delivery or adjuvant for human vaccine. Here, we suggested novel cationic liposome-based mRNA delivery systems called EG-R. EG-R are composed of a cationic lipid, 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and helper lipid.

EG-R can be used for an mRNA delivery carrier. Complexes of Renilla luciferase mRNA and EG-R were intramuscularly injected into mice and observed that the expression of Renilla luciferase was successfully achieved.

Moreover, we prepared a lyophilized form of Renilla luciferase mRNA and EG-R complex and confirmed that the lyophilized form is likely deliver mRNA as efficient as liquid form. Taken together, we suggested the EG-R is efficient mRNA delivery system for various type of mRNA vaccines.

<EG-R can be prepared as a freeze-dried (lyophiliztion) form>

▶Freeze-dried ER-G overcomes many hurdles in vaccine equity through the use of a simple household refrigator (no expensive cryogenic system required)

▶ Conveniently transported, managed and administered with a long shelf life

▶ Relatively inexpensive to distribute to low-income countries 

1. Delivery Efficiency

▶EG-R lipolex observed that the locally expression of Renilla luciferase was successfully achieved.

2. East to Applicate

▶ EG-R lipolex can be applicated infectious disease, Therapeutics for OA and Cancer Vaccines.

3. Biodistribution of EG-R system

▶EG-R lipoplex was observed localized expression pattern of biodistribution.

4. Safety Profile

▶Suspicions grow that nanoparticle(LNP) in some COVID-19 vaccine trigger rare allergic reactions due to PEG hypersensitivity.

▶No issue with Toxicology result.

5. Preclinical Efficacy Profile

▶EG-R demonstrated humoral immunity, CMI and Neutralization activity for SARS-COV-2 WT.

6. Stability at 4℃

▶Lyophilized EG-R lipoplexes at 4℃ for up to 8 weeks show no change in physical characteristics.

7. Long-Term Stabiligy of DP at 4℃

▶The mRNA vaccine complexed with EG-R, DP(EG-COVID) is proved to be very stable stored at 2-8℃ up to 6 months.