MTC/NTM Detection Kit

Real-Time PCR (or Multiplex PCR) based assay system for simultaneous detection of MTC and NTM complex

Pathogen Information

Mycobacterium tuberculosis (MTB) is a pathogenic bacteria species and the causative agent in most cases of tuberculosis. Along with MTB, 5 other species, which we call MTC are regarded as common infectious agents causing tuberculosis. That is why it is necessary to detect MTC, along with MTB. Although NTM is a group of non-tuberculosis related mycobacteria, it usually causes lung infections that mimic tuberculosis resulting in a potential misdiagnosis. Thus, the identification of a single infection or co-infection of MTC and NTM is required for appropriate treatment. 

Product Specification

Detection target MTC (2 species), NTM (10 species)

Detection technology Conventional (End-Point) Multiplex PCR

Specimen type

Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL),

Nasal swab, Oropharyngeal swab, Sputum

Analytical sensitivity 102 - 105 copies

Compatible instruments* ABI Verti thermal Cycler (Applied Biosystems) recommended

PCR running time ~ 1 hr 30 min

* Please inquire us for compatible instrument information before use. 

Product Features

- HotStart PCR system : Ultra high specific and sensitive result

- DnaFree™ system : No host genomic DNA contamination (DiaPlexC™)

- UDG system : No carryover contamination

- Multiplex PCR system : Multiple targets in a single reaction

- Reliable system : Automatic PCR control

- Positive control included

- DNA extraction solution included (Cat. No. SQD21-K100)

- Easy-to-use master mix

- CE certification 

Reference

1. Korean J Clin microbial, Seong Deok Lee, Hye Young Lee, Hyun Chul Kim, Soo Young Kim, Mycobacterium tuberculosis

and Non-Tuberculous Mycobacteria by PCR Assay.

2. Ryan KJ, Ray CG (Editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw-Hill.

3. Sun-Pil Choi, M.D., Bong-Keun Lee M.D1, Jin-Hong Min, M.D. Pathogenic classification and

Clinical Characteristics of Non-Tuberculous Mycobacterial Pulmonary Disease in a National Tuberculosis Hospital. 

Result & Data interpretation

 Ordering Information 

1 DiaPlexQ™ MTC/NTM Detection Kit (w/Ext.), Cat. No. SQD21-K100, includes the kit for the detection of MTC/NTM, and an extension for the pre-treatment of specimens for the PCR and an extraction reagent (DNA Extraction Solution)

2 DiaPlexQ™ MTC/NTM Detection Kit, Cat. No. SQD20-K100 is composed only of the Real-Time Detection Kit for the diagnosis of MTC/NTM.  

MTC/NTM Detection Kit – Ver.3.0

MTC/NTM Detection Kit – Ver.4.0

Real-Time PCR based assay system for simultaneous detection of MTC and NTM complex 

Production Specification 

Product Features

- HotStart PCR system : Ultra high specific and sensitive result

- DnaFree™ system : No host genomic DNA contamination (DiaPlexC™)

- UDG system : No carryover contamination

- Multiplex PCR system : Multiple targets in a single reaction

- Reliable system : Automatic PCR control

- Positive control included

- Easy-to-use master mix

- CE certification  

Reference

1. Korean J Clin microbial, Seong Deok Lee, Hye Young Lee, Hyun Chul Kim, Soo Young Kim, Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria by PCR Assay.

2. Ryan KJ, Ray CG (Editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw-Hill.

3. Sun-Pil Choi, M.D., Bong-Keun Lee M.D1, Jin-Hong Min, M.D. Pathogenic classification and

Clinical Characteristics of Non-Tuberculous Mycobacterial Pulmonary Disease in a National Tuberculosis Hospital. 

Result & Data interpretation 

Ordering Information 

1 SQD21-K100, SQD26-K100, SQD28-K100, includes the kit for the detection of MTC/NTM, and an extension for the pre-treatment of specimens for the PCR and an extraction reagent (DNA Extraction Solution)

2 SQD20-K100, SQD25-K100, SQD27-K100, is composed only of the Real-Time Detection Kit for the diagnosis of MTC/NTM.