Malondialdehyde (MDA) Assay Kit (Cell samples)
Negotiable Min Order Quantity Unit
- Required Quantity
-
- Place of Origin
- U.S.A
- Brand name
- Elabscience
- Payment Terms
- Others,T/T
- Production method
- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Category
- Medical Test Kit
Elabscience Biotechnology,Inc.
U.S.A
- Verified Certificate
-
12
| Product name | Malondialdehyde (MDA) Assay Kit (Cell samples) | Certification | - |
|---|---|---|---|
| Category | Medical Test Kit | Ingredients | - |
| Keyword | assay kit , malondialdehyde assay kit , mda assay kit , mda assay cell samples | Unit Size | - |
| Brand name | Elabscience | Unit Weigh | - |
| origin | U.S.A | Stock | - |
| Supply type | - | HS code | - |
Product Information
Product name: Malondialdehyde (MDA) Assay Kit (Cell samples)
Catalog number: E-BC-K028
Detection instrument: Microplate reader
Valid period: 12 months
Experimental instrument
EP tube, Micropipettor, Microplate reader (530 nm), 96-well plates, Boiling water bath (95℃), Vortex mixer, Centrifuge
Application
This kit can be used to measure the MDA content in animal culture cells and other samples.
Detection significance
The body produce oxygen free radicals through the enzyme system and non-enzyme system, which can attack unsaturated fatty acid on biofilm, leading to lipid peroxidation and forming lipid peroxide, such as aldehyde group(MDA), keto group, hydroxyl group, carbonyl group etc. lipid peroxide can not only transform active oxygen to active chemical, but also enhance the effect of active oxygen. Detection of the content for MDA can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.
Detection principle
Malonyldialdehyde (MDA) in catabolite of Lipid peroxide (LPO) can condensate with thiobarbituric acid (TBA) and form red compound, which has the maximum absorbance peak at 532 nm.
Operation table
| Blank tube | Standard tube | Sample tube |
Ethanol (μL) | 100 |
|
|
10 nmol/mLstandard solution (μL) |
| 100 |
|
Test sample (μL) |
|
| 100 |
Working solution (μL) | 1000 | 1000 | 1000 |
Cover the tubes, mix with vortex mixers, mix with vortex mixers and incubate for 40 min at above 95℃. Cool the tubes with running water. Centrifuge for 10 minutes at 4000 rpm. Measure the OD value of empty microtiter plate at 530 nm wavelength. Accurately add 0.25 mL reaction solution of each tube into a new 96-well plate, then measure the OD value of each well (minus the empty plate reader when calculating).
B2B Trade
| Price (FOB) | Negotiable | transportation | - |
|---|---|---|---|
| MOQ | Negotiable | Leadtime | Negotiable |
| Payment Options | Others,T/T | Shipping time | Negotiable |
- President
- Yibing Leng
- Address
- 14780 Memorial Drive, Suite 216, Houston, Texas 77079.
- Product Category
- Beauty & Personal Care,Industrial Supplies,Medical Test Kit
- Year Established
- 2011
- No. of Total Employees
- 101-500
- Company introduction
-
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.
Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits，Biochemical Kits and Other Reagents.
Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.
- Factory Information
-
Elabscience Biotechnology,Inc.
- Main Product
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