ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit

ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
U.S.A
Brand name
Elabscience
Payment Terms
Others,T/T
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
elisa kit, st elisa kit, 5-ht elisa kit, 5-hydroxytryptamine elisa kit
Category
Medical Test Kit

Elabscience Biotechnology,Inc.

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2011
Business type
Manufacturer
Verified Certificate

12

Product name ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit Certification -
Category Medical Test Kit Ingredients -
Keyword elisa kit , st elisa kit , 5-ht elisa kit , 5-hydroxytryptamine elisa kit Unit Size -
Brand name Elabscience Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

 

Product name: ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit


Catalog number: E-EL-0033


Size: 96T

Reactivity: Universal

Detection Range: 15.63~1000 ng/mL

Sensitivity: 9.38 ng/mL

Lead time: 7~10days

 

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  ST/5-HT concentrations in serum, plasma and other biological fluids.

 

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ST/5-HT. During the reaction,  ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Specificity

This kit recognizes some recombinant and natural ST/5-HT. No significant cross-reactivity or interference was observed.

 

Precision 

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ST/5-HT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ST/5-HT were tested on 3 different plates, 20 replicates in each plate.

 

  

Kit components & Storage 

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 10 mL
HRP Conjugate Diluent1 vial, 10 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

 

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

 

 

Citations

1.Publication: Hussein A M, Abbas K M, Abulseoud O A, et al. Effects Of Ferulic Acid On Oxidative Stress, Heat Shock Protein 70, Connexin 43, And Monoamines In The Hippocampus Of Pentylenetetrazole-Kindled Rats[J]. Canadian Journal of Physiology And Pharmacology, 2017.

Species: Rat
Sample Type: tissue homogenates

2.Publication: Özgür B G, Aksu H, Birincioğlu M, et al. Antidepressant-Like Effects Of The Xanthine Oxidase Enzyme Inhibitor Allopurinol In Rats. A Comparison With Fluoxetine[J]. Pharmacology Biochemistry and Behavior, 2015, 138: 91-95.
Species: Rat
Sample Type: blood

3.Publication: Bi Q R, Hou J J, Qi P, et al. Venenum Bufonis Induces Rat Neuroinflammation By Activiating NF-κB Pathway And Attenuation Of BDNF[J]. Journal of Ethnopharmacology, 2016, 186: 103-110.
Species: Rat
Sample Type: tissue homogenates

4.Publication: Jiang S H, Li J, Dong F Y, et al. Increased Serotonin Signaling Contributes To The Warburg Effect In Pancreatic Tumor Cells Under Metabolic Stress And Promotes Growth Of Pancreatic Tumors In Mice[J]. Gastroenterology, 2017, 153: 277-291.
Species: Universal
Sample Type: culture supernatant tissue homogenates and plasma


B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Others,T/T Shipping time Negotiable

Elabscience Biotechnology,Inc.

Country / Year Established
U.S.A U.S.A / 2011
Membership
BIZ
Business type
Manufacturer

12

President
Yibing Leng
Address
14780 Memorial Drive, Suite 216, Houston, Texas 77079.
Product Category
Beauty & Personal Care,Industrial Supplies,Medical Test Kit
Year Established
2011
No. of Total Employees
101-500
Company introduction
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.


Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling KitsBiochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions. 

 

Factory Information

Elabscience Biotechnology,Inc.

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