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Human IL-2(Interleukin 2) ELISA Kit

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Place of Origin: U.S.A

Brand name: Elabscience

Payment Terms: Others,T/T

Production method: Negotiable

Shipping / Lead Time: Negotiable / Negotiable

Keyword: elisa kit, human il-2 elisa kit, il-2 elisa kit, interleukin 2 elisa kit

Category: Medical Test Kit

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Elabscience Biotechnology,Inc.

Membership: BIZ

Country / Year Established: U.S.A / 2011

Business type: Manufacturer

Verified Certificate: 12

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PRODUCT DESCRIPTION COMPANY INFORMATION Related Products

Product name	Human IL-2(Interleukin 2) ELISA Kit	Certification	-
Category	Medical Test Kit	Ingredients	-
Keyword	elisa kit , human il-2 elisa kit , il-2 elisa kit , interleukin 2 elisa kit	Unit Size	-
Brand name	Elabscience	Unit Weigh	-
origin	U.S.A	Stock	-
Supply type	-	HS code	-

Product Information

Product name: Human IL-2(Interleukin 2) ELISA Kit

Catalog number: E-EL-H0099

Size: 96T

Reactivity: Human

Detection Range: 125.00~8000 pg/mL

Sensitivity: 75.00 pg/mL

Lead time: 7~10days

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human IL-2 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2. The concentration of Human IL-2 in samples can be calculated by comparing the OD of the samples to the standard curve.

Specificity

This kit recognizes some recombinant and natural Human IL-2. No significant cross-reactivity or interference was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Human IL-2 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human IL-2 were tested on 3 different plates, 20 replicates in each plate.

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 10 mL
HRP Conjugate Diluent	1 vial, 10 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

Citations

1.Publication: Zeng Z P, Wang K, Li Y Y, et al. Down-Regulation Of MicroRNA-451a Facilitates The Activation And Proliferation Of CD4+ T Cells By Targeting Myc In Patients With Dilated Cardiomyopathy[J]. The Journal of Biological Chemistry, 2016.

Species: Human

Sample Type: culture supernatant

2.Publication: Li W, Dong H, Huang Y, et al. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients[J]. Plos Neglected Tropical Diseases, 2016.

Species: Human

Sample Type: culture supernatants and serum

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Price (FOB)	Negotiable	transportation	-
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Elabscience Biotechnology,Inc.

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Country / Year Established: U.S.A / 2011

Membership: BIZ

Business type: Manufacturer

President: Yibing Leng

Address: 14780 Memorial Drive, Suite 216, Houston, Texas 77079.

Product Category: Beauty & Personal Care,Industrial Supplies,Medical Test Kit

Year Established: 2011

No. of Total Employees: 101-500

Company introduction: <p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.

Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits，Biochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.