High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. Fundamentally, chromatography is a technique used to separate the components contained in a sample. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures. The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. HPLC utilized the quality and quantity of both small molecules and polymeric species. High Performance Liquid Chromatography (HPLC) Analysis Services at Creative-Proteomics offers you a state-of-the-art liquid separations platform that includes standard HPLC with frequently used separation mechanisms.