FB Plasmid Miniprep Kit                                                                   

For research use only                                                                                                                                     

Sample: up to 4 ml bacterial cells                                                             Yield: up to 30μg of plasmid

Introduction                                                                                                                                                                             

The FB Plasmid Miniprep Kit provides a fast, simple, and cost-effective plasmid miniprep method for isolation of plasmid DNA from cultured bacterial cells. The FB Plasmid Miniprep Kit is based on alkaline lysis of bacterial cells followed by binding of DNA onto the glass fiber matrix of the spin column in the presence of high salt (1, 2).Phenol extraction and ethanol precipitation are not required, and high-quality plasmid DNA is eluted in a small volume of Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). Plasmid DNA purified with FB Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, Sequencing, library screening, in vitro translation, transfection of robust cells,ligation and transformation. The entire procedure 
can be completed within 15-20 minutes.