Finding full-length target sequences is a key objective of many library screens. Our novel full-length cDNA synthesis procedure combines a proprietary process for reducing RNase H reverse transcriptase activity with unique techniques for mRNA isolation, 5´ cap full-length enrichment, and reduction of oligo(dT) priming. Together, these steps greatly enhance the likelihood of constructing a library with full length inserts, giving you better targets at which to aim.

Full length-enriched cDNA is usually produced using modified template-switching approach. The method combines cDNA synthesis and amplification and results in representative cDNA population enriched with full-length sequences even from small amount of starting materials.

The method combines cDNA synthesis and amplification and allows generating representative cDNA populations enriched with full-length sequences even from small amounts of starting material. After cDNA synthesis and amplification, double stranded cDNA is normalized using DSN-normalization method.

The method combines cDNA synthesis and amplification and results in representative cDNA population enriched with full-length sequences even from small amounts of starting materials.After cDNA synthesis, the double stranded cDNA is depleted using a Duplex-Specific Nuclease (DSN)-based method. The method allows specific removal of selected transcripts without loss of average cDNA size.

To further enhance the chances of identifying full-length inserts, we have combined our proprietary full-length library procedure with the Gateway® recombination-based cloning technology. Using Gateway® technology allows us to avoid restriction enzyme digestion of the cDNA during library construction. Stringent quality control ensures that libraries are highly enriched for full-length cDNA clones. Our full-length cDNA libraries routinely achieve:

•    65–85% full-length clones
•    >1 x 10⁷ clones .
•    Insert lengths up to 8 kb.