cDNA Library Construction Service

cDNA Library Construction Service


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
U.S.A
Brand name
CD Genomics
Payment Terms
Negotiable
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
sanger sequencing, cdna, 454 sequencing, cdna sequencing
Category
Pharmaceutical Intermediates

CD Genomics

Membership
BIZ
Country / Year Established
U.S.A U.S.A /
Business type
Manufacturer
Verified Certificate

10

Product name cDNA Library Construction Service Certification -
Category Pharmaceutical Intermediates Ingredients -
Keyword sanger sequencing , cdna , 454 sequencing , cdna sequencing Unit Size -
Brand name CD Genomics Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

CD Genomics has extensive experience in constructing high quality cDNA libraries in a vector of choice. The cDNA libraries are cloned into a standard high-copy vector, picked and arrayed into either 96-well or 384-well microtitre plates amenable to most high throughput DNA sequencing production systems. The libraries are well-suited for EST sequencing and other gene discovery applications. CD Genomics can also sequence ESTs from cDNA libraries via Sanger sequencing or 454 sequencing.  

 

The steps of cDNA library construction
 

  • Extraction of RNA from a variety of samples
  • Generation of phage-or plasmid-based cDNA libraries from size-selected cDNA
  • Directional cloning of cDNAs into any vector
  • Production of a library with at least 106 cfu
  • Library amplification to generate higher titer library
  • Library delivered on plates, filters or glycerol stock
  • Verification of library quality by randomly picking clones and determining insert sizes by restriction digest
  • Verification of library complexity by DNA sequencing of the inserts from a subset of randomly picked clones (Optional)

 

 

B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Negotiable Shipping time Negotiable

CD Genomics

Country / Year Established
U.S.A U.S.A /
Membership
BIZ
Business type
Manufacturer

10

President
Mr. Zhang
Address
45-1 Ramsey Road, Shirley, NY 11967,USA
Product Category
Petrochemical Products
No. of Total Employees
101-500
Company introduction

We are aiming at providing the research community with high quality manual DNA sequencing  services. Due to the demand for our services has increased, CD Genomics has already updated its technology platform  to automated ABI instruments. At present, our senior bioinformaticians have ever viewed more than ten thousands of trace files and accumulated abundant experience with our 3730 and 3730XL analyzers. We continue to work hard to offer you the same dependable services to pharmaceutical and biotech companies, as well as academia and government agencies for the purpose of satisfying all your sequencing needs.

 

In 2007, CD Genomics has expanded its services to molecular biology research and its development needs. we have become a service provider in genotyping,library construction,and mutagenesis analyses. Our progress can not be achieved without our large group of client's support.

 

Through nearly ten year's hard working and depend on our professional work team, we are proud of satisfying the needs of our clients both at home and abroad, which across more than 50 countries and districts. We always devote ourselves to providing you with the best and professional service.

 

 

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