cDNA Library Construction Service
Negotiable Min Order Quantity Unit
- Required Quantity
-
- Place of Origin
- U.S.A
- Brand name
- CD Genomics
- Payment Terms
- Negotiable
- Production method
- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Keyword
- sanger sequencing, cdna, 454 sequencing, cdna sequencing
- Category
- Pharmaceutical Intermediates
CD Genomics
U.S.A
- Verified Certificate
-
10
| Product name | cDNA Library Construction Service | Certification | - |
|---|---|---|---|
| Category | Pharmaceutical Intermediates | Ingredients | - |
| Keyword | sanger sequencing , cdna , 454 sequencing , cdna sequencing | Unit Size | - |
| Brand name | CD Genomics | Unit Weigh | - |
| origin | U.S.A | Stock | - |
| Supply type | - | HS code | - |
Product Information
CD Genomics has extensive experience in constructing high quality cDNA libraries in a vector of choice. The cDNA libraries are cloned into a standard high-copy vector, picked and arrayed into either 96-well or 384-well microtitre plates amenable to most high throughput DNA sequencing production systems. The libraries are well-suited for EST sequencing and other gene discovery applications. CD Genomics can also sequence ESTs from cDNA libraries via Sanger sequencing or 454 sequencing.
The steps of cDNA library construction
- Extraction of RNA from a variety of samples
- Generation of phage-or plasmid-based cDNA libraries from size-selected cDNA
- Directional cloning of cDNAs into any vector
- Production of a library with at least 106 cfu
- Library amplification to generate higher titer library
- Library delivered on plates, filters or glycerol stock
- Verification of library quality by randomly picking clones and determining insert sizes by restriction digest
- Verification of library complexity by DNA sequencing of the inserts from a subset of randomly picked clones (Optional)
B2B Trade
| Price (FOB) | Negotiable | transportation | - |
|---|---|---|---|
| MOQ | Negotiable | Leadtime | Negotiable |
| Payment Options | Negotiable | Shipping time | Negotiable |
- President
- Mr. Zhang
- Address
- 45-1 Ramsey Road, Shirley, NY 11967,USA
- Product Category
- Petrochemical Products
- No. of Total Employees
- 101-500
- Company introduction
-
We are aiming at providing the research community with high quality manual DNA sequencing services. Due to the demand for our services has increased, CD Genomics has already updated its technology platform to automated ABI instruments. At present, our senior bioinformaticians have ever viewed more than ten thousands of trace files and accumulated abundant experience with our 3730 and 3730XL analyzers. We continue to work hard to offer you the same dependable services to pharmaceutical and biotech companies, as well as academia and government agencies for the purpose of satisfying all your sequencing needs.
In 2007, CD Genomics has expanded its services to molecular biology research and its development needs. we have become a service provider in genotyping，library construction，and mutagenesis analyses. Our progress can not be achieved without our large group of client's support.
Through nearly ten year's hard working and depend on our professional work team, we are proud of satisfying the needs of our clients both at home and abroad, which across more than 50 countries and districts. We always devote ourselves to providing you with the best and professional service.
- Main Product
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