Rat ADP/Acrp30 Elisa kit

Catalog No: E-EL-R0329

Elabscience Biotechnology Co., Ltd.

Package: 96T/kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma and other biological fluids.

Detection Range

1.56 to 100 ng/mL.

Sensitivity

The minimum detectable dose of RatADP/Acrp30 is 0.94 ng/mL.

Specificity

This kit recognizes natural and recombinantRat ADP/Acrp30. No significant cross reactivity or interference between Rat ADP/Acrp30 and analogues was observed.

Repeatability  

Coefficient of variation were<10%.

Test principle

This ELISA kit uses Sandwich ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to ADP/Acrp30. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADP/Acrp30 and Avidin Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADP/Acrp30, biotinylated detection antibody and Avidin HRP conjugate will appear blue in color. The enzyme substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ADP/Acrp30. You can calculate the concentration of ADP/Acrp30 in the samples by comparing the O.D. of the samples to the standard curve.