Rat IFN-γ (Interferon Gamma) ELISA Kit

Catalog No:E-EL-R0009

Elabscience Biotechnology Co., Ltd.

Price:495USD

Package: 96T/kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.

Detection Range

31.25~2000pg/mL

Sensitivity

The minimum detectable dose of Human FABP5 is 18.75pg/mL

Specificity

This kit recognizes natural and recombinant Human CD59. No significant cross reactivity or interference between Human CD59 and analogues was observed.

Repeatability

Coefficient of variation were<10%.

Test principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-γ. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IFN-γ. You can calculate the concentration of IFN-γ in the samples by comparing the OD of the samples to the standard curve.