Human β-actin (Actin Beta) ELISA Kit

Catalog No:E-EL-H0998

Elabscience Biotechnology Co., Ltd.

Price: 580USD

Package: 96T/kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Human β-actin concentrations in serum, plasma and other biological fluids.

Detection Range

0.16 to 10ng/mL.

Sensitivity

The minimum detectable dose of Human β-actin is 8.9999999999999997E-2ng/mL

Specificity

This kit recognizes natural and recombinant Human β-actin. No significant cross reactivity or interference between Human β-actin and analogues was observed.

Repeatability

Coefficient of variation were<10%.

Test principle

This ELISA kit uses SandwichELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to β-actin. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for β-actin and AvidinHorseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain β-actin, biotinylated detection antibody and AvidinHRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of β-actin. You can calculate the concentration of β-actin in the samples by comparing the O.D. of the samples to the standard curve.