Intended Use

Cervical carcinoma is closly associated with Human Papillomavirus. The presence of HPV in female genital tract is associated with a number of diseases, neoplasia and cancer on genital tract.

The types 16/18/31/33/35/39/45/51/52/53/56/58/59/66/68 are considered to belong to group that puts patient infected with these types at high-risk for cervical carcinogenesis.

CBG high-risk fifteen subtypes HPV-DNA qualitative Assay Kit (CBG HPV-DNA qualitative assay) is an in vitro nucleic acid capture hybridization assay with multi-probes using microplate colorimetry method for the direct qualitive detection of thirteen high-risk types Human papilloma virus in cervical specimens .

The HPV types detected by this assay are the high-risk types 16/18/31/33/35/39/45/51/52/53/56/
58/59/66/68. This assay cannot determine the specific HPV genotype present.

Principle of the product

The Principle of the product is an in vitro nucleic acid capture hybridization assay with multi-probes using microplate colorimetry method. the biotin labeled HPV-DNA fragment was made from extracted genomic DNA by Amplifying .

The labeled DNA fragment hybridize with multi-specific probes in microplate, and the Enzyme bound with probes reacts substrate. The strength of developing color is measured as optical density (O.D) on ELISA reader(colorimeter)

Features & Advantage points of product

• Capture Hybridization with multi-probes using colorimetry
• Can use random automatic ELISA system through the optimization of reacting temperature
• High accuracy & good reproducibility
• Decreasing of error through the simple process
• The HPV types detected by this assay are the high-risk types
  16/18/31/33/35/39/45/51/52/53/56/58/59/66/68.
• This assay cannot determine the specific HPV genotype present.